Characterization of the cyclin-dependent kinase 6 gene in Apis cerana cerana in response to multiple environmental stresses.
نویسندگان
چکیده
Cyclin-dependent kinases (CDKs) are serine/threonine kinases that play critical roles in the cell cycle regulation. Herein, we describe the identification of a CDK gene from Apis cerana cerana, named AccCDK6. The full-length cDNA is 1778 bp long, including an ORF of 1380 bp that encodes a polypeptide of 459 amino acid residues. Multiple sequence alignment analysis showed that the predicted AccCDK6 sequence shares a high similarity with CDK6 genes of other species, and this protein may share an evolutionary predecessor with Drosophila CDK4. The expression patterns of the gene were also analysed, and the transcript was detected throughout the larval, pupal, and adult developmental stages. Furthermore, the expression level of the mRNA of the gene in adult workers was influenced by H2O2, ultraviolet (UV) light, temperature (42 degrees C), HgCl2, and pyriproxyfen. These results indicate that AccCDK6 responds to multiple environmental stresses and may also participate in intracellular reactions of reactive oxygen species (ROS) and development processes in honey-bees.
منابع مشابه
Characterization of a Decapentapletic Gene (AccDpp) from Apis cerana cerana and Its Possible Involvement in Development and Response to Oxidative Stress
To tolerate many acute and chronic oxidative stress-producing agents that exist in the environment, organisms have evolved many classes of signal transduction pathways, including the transforming growth factor β (TGFβ) signal pathway. Decapentapletic gene (Dpp) belongs to the TGFβ superfamily, and studies on Dpp have mainly focused on its role in the regulation of development. No study has inve...
متن کاملEvidence of Apis cerana Sacbrood virus Infection in Apis mellifera.
Sacbrood virus(SBV) is one of the most destructive viruses in the Asian honeybee Apis cerana but is much less destructive in Apis mellifera In previous studies, SBV isolates infecting A. cerana(AcSBV) and SBV isolates infecting A. mellifera(AmSBV) were identified as different serotypes, suggesting a species barrier in SBV infection. In order to investigate this species isolation, we examined th...
متن کاملCharacterization and Mutational Analysis of Omega-Class GST (GSTO1) from Apis cerana cerana, a Gene Involved in Response to Oxidative Stress
The Omega-class of GSTs (GSTOs) is a class of cytosolic GSTs that have specific structural and functional characteristics that differ from those of other GST groups. In this study, we demonstrated the involvement of the GSTO1 gene from A. cerana cerana in the oxidative stress response and further investigated the effects of three cysteine residues of GSTO1 protein on this response. Real-time qu...
متن کاملSequencing and Expression Characterization of Antifreeze Protein Maxi-Like in Apis cerana cerana
Antifreeze proteins (AFPs) are biological cryoprotectants with unique properties that play a crucial role in regulating the molecular mechanisms governing cold resistance in insects. To identify and characterize Apis cerana cerana AFP (AcerAFP), we cloned the full-length cDNA of AcerAFP and examined its expression patterns in A. cerana cerana. A nucleotide alignment analysis showed that the ent...
متن کاملTranscriptomic analysis to uncover genes affecting cold resistance in the Chinese honey bee (Apis cerana cerana)
The biological activity and geographical distribution of honey bees is strongly temperature-dependent, due to their ectothermic physiology. In China, the endemic Apis cerana cerana exhibits stronger cold hardiness than Western honey bees, making the former species important pollinators of winter-flowering plants. Although studies have examined behavioral and physiological mechanisms underlying ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Zeitschrift fur Naturforschung. C, Journal of biosciences
دوره 67 5-6 شماره
صفحات -
تاریخ انتشار 2012